E-Book

E-Book 2nd Congress

  • Examination of Bocavirus and Tusavirus genomes in celiac patients and negative controls
  • Shahnaz Mohamadi,1,* Seyed Reza Mohebbi,2 Seyed Masoud Hosseini,3 Piruz Shadbash,4
    1. Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
    2. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
    3. Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
    4. Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.


  • Introduction: During the last two decades, the rate of diagnosis of celiac disease or gluten-sensitive enteropathy has been increasing significantly in most western countries and also in our country. According to advancing research, in addition to genetic factors, environmental factors (viral infections) also play a role in the development of celiac disease. Viral infections cause intestinal damage and the destruction of intestinal villi. Among the viruses studied in celiac disease are human Bocavirus and Tusavirus. Bocavirus and human Tusavirus belong to the family of viruses called Parvoviridae. The viruses of this family are small and do not have an envelope, they have a 20-sided capsid symmetry and a linear single-stranded DNA genome with an approximate length of 4-6 kilobytes. Objectives: Studies on the identification and frequency of viral gastrointestinal infections in Iran are relatively limited, considering the diversity of factors that cause celiac disease and the identification of new viruses, it is necessary to conduct more studies. Therefore, the current study is to add more information about Boca viruses. And Tusa and the effect they have on this disease have been done.
  • Methods: 161 stool samples, including 101 controls and 60 patients, were collected from diarrheal patients in Khorramabad and transferred to the Gastroenterology and Liver Research Center Hospital of Taleghani Hospital in Tehran. DNA was extracted from them, and then Nested PCR method was performed to check and confirm Boca and Tusa viruses. After observing the desired band, the samples were analyzed to determine the sequence.
  • Results: The statistical population included 94 women (58.4%) and 67 men (41.6%), and 2 positive samples were found with Bocavirus and Tusavirus genomes.
  • Conclusion: The results show the role of bocavirus in celiac gastrointestinal patients and also the role of Tusavirus in these patients. Despite the lower prevalence of these viruses compared to developed countries, it is recommended to improve public health education and health care.
  • Keywords: human Bocavirus, human Tusavirus, celiac disease