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  • Prevalence of enterotoxin gene in MRSA isolated from clinical samples in Shahrekourd
  • Parisa behshood,1,* Samane ansarinia,2 Elahe Tajbakhsh,3
    1. Phd Department of Microbiology,Young Researchers and Elite Club , Shahrekord Branch , Islamic Azad University Shahrekord, Iran
    2. Master Department of Microbiology,Young Researchers and Elite Club , Shahrekord Branch , Islamic Azad University Shahrekord, Iran
    3. PhD, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran


  • Introduction: Staphylococcus aureus is a ubiquitous bacterium that has plagued humans for thousands of years. The aim Of this Study is to enterotoxin genes of Staphylococcus aureus isolated from clinical samples in Isfahan
  • Methods: During one one-year period, a total of 100 clinical samples isolated from nosocomial infections were studied. Isolates were identified by standard methodology in the microbiology laboratory.MRSA isolates had been isolated by using the agar screening method. The pattern of antimicrobial resistance isolated strains was determined by the standard disk diffusion method. DNA of the isolates was extracted by DNA extraction kit (Fermentas) according to the manufacturer's protocol. Amplification of seg and sea enterotoxin genes was done by a specific primer and polymerase chain reaction method.
  • Results: Among the 100 Staphylococcus aureus isolates recovered, 65 isolates (65%) were MRSA. The frequency of antimicrobial patterns MRSA isolates showed that isolates, 100% to penicillin and oxacillin, 80% to nitrofurantoin, 63% to tetracycline, 58.46%to erythromycin, 46.15%to gentamicin,33.8%to clindamycin,35.38%to cotrimoxazole andto ciprofloxacin 26.15% were resistant. However, none of the MRSA isolates were resistant to vancomycin. Also prevalence of the enterotoxin type (g) gene was %38.56 and enterotoxin type (a) % 80 in MRSA strains
  • Conclusion: results of this study showed that a high percentage of Staphylococcus aureus clinically isolates produced enterotoxin. Considering that these toxins are superantigen and can more intense the complications of nosocomial infections. detecting and rapid treatment of these infections is essential.
  • Keywords: staphylococcus aureus- Antibiotic resistance-clinical samples