E-Book

E-Book 3rd Congress

  • Investigating the effect of ethyl acetate and n-butanol fractions of the hydro alcoholic extract of Ferula assa-foetida plant stem on the process of angiogenesis of the chorioallantoic membrane of chick embryos
  • Mohammad Reza Pour Mohammad,1 Jina Khayat Zadeh,2,* Ahmad Zahani,3 Maryam Tehranipour,4
    1. Department of Medical Parasitology, Faculty of Paramedicine, Ilam University of Medical Sciences, Ilam, Iran
    2. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
    3. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
    4. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran


  • Introduction: Angiogenesis refers to the biological process of sprouting new vessels from existing vessels in the tissue. Angiogenesis is a physiological process that is highly regulated and occurs in cases such as wound healing, menstrual cycles, placental growth, and ovulation. Ferula assa-foetida is one of the prominent medicinal plants in traditional medicine. This research was conducted with the aim of investigating the effect of ethyl acetate and n-butanol fractions of the hydroalcoholic extract of Ferula assa-foetida on the process of angiogenesis of the chorioallantoic membrane of chick embryos.
  • Methods: In this research, 72 Ross spray eggs were randomly divided into 8 groups including control, laboratory control (pbs) and 6 experimental groups. After incubation, on the second day of the window, an egg was created and in on the eighth day, after placing the gelatin sponge on the chorioalantoic curve, the fractions of the ferula assa-foetida stem extract (100, 200 and 300 μg/ml) were injected onto the chorioalantoic membrane of the chick embryo. On the twelfth day, the corioalantoic membrane was taken and length, The number of vascular splits, weight and height of the embryos were measured. The collected data were analyzed by Excel and SPSS 20 statistical software.
  • Results: The average number and total length of vascular branches in the laboratory control group did not show any significant difference compared to the control group. The average number and length of vascular branches in concentrations of 100, 200, 300 µg/ml of ethyl acetate and n-butanol fractions of Ferula assa-foetida showed a significant decrease compared to the control group.
  • Conclusion: According to the methods of this research, the above fractions from the stem of Ferula assa-foetida have an inhibitory effect on angiogenesis in the chorioallantoic membrane of chick embryos. Also, it seems that the compounds in Ferula assa-foetida can be used to inhibit angiogenesis in cancerous tissues.
  • Keywords: Angiogenesis, Ferula assa-foetida, Chorioalantoic Membrane, Chick Embryo