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  • Investigating the effect of ethyl acetate and n-butanol fractions of the hydro alcoholic extract of Ferula assa-foetida plant stem on the expression of FGF and VEGF genes in chicken embryos.
  • Mohammad Reza Pour Mohammad,1 Jina Khayat Zadeh,2,* Ahmad Zahani,3 Maryam Tehranipour,4
    1. Department of Medical Parasitology, Faculty of Paramedicine, Ilam University of Medical Sciences, Ilam, Iran
    2. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
    3. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
    4. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran


  • Introduction: FGF produced by endothelial cells induces proliferation and increases the survival of endothelial cells. FGF are collagenase activators and lead to the sprouting of new vessels from blood vessels, FGF is needed to maintain and progress angiogenesis. It is believed that VEGF acts in the beginning of angiogenesis. Ferula assa-foetida is one of the prominent medicinal plants in traditional medicine. This research was conducted with the aim of investigating the effect of ethyl acetate and n-butanol fractions of the hydroalcoholic extract of the stem of Ferula assa-foetida plant on the expression of FGF and VEGF genes in chick embryos.
  • Methods: In this research, 72 Ross spray eggs were randomly divided into 8 groups including control, laboratory control (pbs) and 6 experimental groups. After incubation, on the second day of the window, an egg was created and in on the eighth day, after placing the gelatin sponge on the chorioalantoic curve, the fractions of the ferula assa-foetida stem extract (100, 200 and 300 μg/ml) were injected onto the chorioalantoic membrane of the chick embryo. In order to extract RNA and examine gene expression, a sample was taken from the chorioallantoic membrane and by making cDNA, the expression changes of VEGF and FGF genes were quantitatively measured. The collected data were analyzed by Excel and SPSS 20 statistical software.
  • Results: The average expression of VEGF and FGF genes in the laboratory control group did not show any significant difference compared to the control group. The average expression of VEGF and FGF genes in the concentrations of 100, 200, 300 µg/ml of ethyl acetate and n-butanol fractions of ferula assa-foetidastem showed a significant decrease compared to the control group.
  • Conclusion: According to the methods of this research, the above fractions from the stem of Ferula assa-foetida have an inhibitory effect on angiogenesis in the chorioallantoic membrane of chick embryos. Also, it seems that the compounds in Ferula assa-foetida can be used to inhibit angiogenesis in cancerous tissues.
  • Keywords: gene expression, Ferula assa-foetida, Chorioallantoic Membrane, Chick Embryo