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  • Identification of potential biomarkers related to pancreatic ductal adenocarcinoma in a bioinformatics study
  • AmirReza Homaei,1,*
    1. Medical Genomics Research Center, Islamic Azad Tehran Medical Sciences University, Tehran, Iran


  • Introduction: Pancreatic cancer is one of the most common causes of death in cancer patients. This cancer has different types, the most common type of which is called pancreatic ductal adenocarcinoma (PDAC). PDAC has a 5-year survival rate of less than 10%. Our aim of conducting the following research is to investigate and identify mRNA and miRNA biomarkers in PDAC pancreatic cancer
  • Methods: this study method is based on the analysis of Protein-Protein interaction network and mRNA - miRNA interactions. To conduct this study, the Microarray gene-expression data profile containing tumor samples, adjacent non-tumor tissue and normal pancreas donors was selected. After bioinformatics analysis, up-regulated and down-regulated genes were selected and the protein protein interaction network was drawn for them. After analysis using software, one gene module and one hub gene were selected from each set of up-regulated and down-regulated genes. In the next step, common genes between modules and hub genes were obtained for each of the up-regulated and down-regulated gene sets. Then, using web tools, validation was done for each of the obtained common genes, and finally KRT19 gene was selected as the only valid gene introduced by online bioinformatics tools. In the next step, the interaction between mRNA and miRNA was investigated by bioinformatics tools and among the introduced miRNAs, two miRNAs(hsa-miR-193b-3p and hsa-let-7b-5p) with significant survival effect on patients were selected as potential biomarkers for the KRT19 gene. In the last step, targets scan was performed for the two selected miRNAs and it was found that hsa-miR-193b-3p and hsa-let-7b-5p have 332 and 990 other target genes, respectively, except KRT19
  • Results: The final result is that three potential biomarkers include KRT19 gene, hsa-miR-193b-3p and hsa-let-7b-5p as potential biomarkers obtained from this study
  • Conclusion: Other studies, including immunohistochemical studies conducted by other researchers in the past, have investigated and proved the relationship of KRT 19 gene with metastasis and poor prognosis in PDAC
  • Keywords: PDAC/systems biology/cancer/biomarker/tumor marker