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  • Evaluation of antimicrobial effects of bifidobacterium bifidum on some pathogenic microorganisms
  • Samaneh Ansarinia,1,* Parisa Behshood,2 Elahe Tajbakhsh,3 Faezeh Amiri,4
    1. Master Department of Microbiology, Young Reasearchers and Eliet Club, Shahrekord Branch, Islamic Azad University Shahrekord, Iran
    2. Phd Department of Microbiology, Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University Shahrekord, Iran
    3. Associate Professor, Biology - Microbiology, Basic Biology Sciences,
    4. Biotechnology Bachelor, Naghsh Jahan Isfahan non profit organization


  • Introduction: Branched bifidobacteria, rod-shaped, non-motile, anaerobic, Gram-positive, B They are aerobic and catalase negative and belong to the Bifidobacteriaceae family and the Actinobacteria branch. The genus Bifidobacterium currently includes more than 90 species, excluding taxa Unclassified Bifidobacterium first fed from infant's feces, separated from mother, but fed to infants Various ecological niches including fermented milk and anaerobic digestion facilities have been discovered However, the most common isolates are from the gastrointestinal tract of humans and animals. Conditions Growth (eg, temperature, pH, oxygen level) of bifidobacteria significantly varied among strains. It is not different (3). (Ruiz et al., 2011). For example, the optimal growth temperature is between 36 and 38, respectively °C and 41 to 43 °C for strains isolated from humans and animals. In addition, the optimum pH for growth is around pH 6.5-7.0, where B. animalis and B. thermacidophilum are also metabolically active at pH 3.5-4.0. Probiotics are live microbial food supplements that have beneficial effects on health have consumers This group of bacteria plays an important role in preventing infection in the parts They play a role in various parts of the body, especially the digestive system. Among probiotic microorganisms, bacteria The genus Bifidiobacterium 6 is known as the most common organism in the production of probiotic products have been introduced. These bacteria are gram positive, catalase negative and have no spores and have characteristics such as Tolerance of bile acids, production of organic acids, antimicrobial compounds and cell aggregation Pathogenic bacteria and preventing the attachment of pathogenic bacteria to the surfaces of the digestive system Are. As a result of the use of probiotic supplements, beneficial colonies will be created that can Like natural floromicrobials, prevent pathogenic bacteria from reaching and attaching to the target cell destroy them by producing antimicrobial compounds and prevent the onset of the disease process and at the same time Now provide the conditions for the natural floromicrobe to repair and restore itself, then the colonies They will be replaced by the natural bacterial environment that has regenerated itself. One of the prerequisites of becoming a probiotic strain is the ability to colonize in one place. Specific, for example, is in the gastrointestinal tract, so that probiotic strains can effectively interact with the host and the host microbiome. Colonization of probiotics on some pathogens It excels and thus gives the host protection against pathogenic infections. Studies Different species showed that different species of Bifidobacteria have anti-infection properties. B. longum ATCC 15708 Antimicrobial activity against many pathogens including Escherichia, Salmonella typhimurium and Listeria monocytogenes were indicated. B. longum BB536. Against intestinal sepsis caused by Pseudomonas aeruginosa, possibly from Protects the intestinal epithelial cells by interfering with the adhesion of pathogens (8). BB536 Probably through the modulation of the gut microbiota، that is، increasing the abundance of the genus Faecalibacterium، Improves upper respiratory infections in healthy preschool children. Also, one A randomized, double-blind, placebo-controlled trial shows that the administration of BB536 in Combination with standard triple treatment (osmeprazole, amoxicillin, clarithromycin) eradication rate Improves Helicobacter pylori infection in 63 patients. Prescription of B. lactis BB-12 in early adulthood Childhood Reduces Respiratory Tract Infections. Was. B. animalis AHC7 to protect mice It was found against S. typhimurium infection and prevents acute diarrhea in dogs. Mechanisms Basic Prevention of Acute Diarrhea B. animalis AHC7 due to weakened activation of pre-transcription factor Inflammation is in response to infection. Bifidobacteria also latent in the transport of infectious strains And chronically they are used. For example, B. longum ATCC 15707 can cause infection Prevent Clostridium difficiles. While B. longum 51A protects against lung infection caused the Klebsiella pneumoniae This protection granted by B. longum 51A due to activation The signaling pathway is a Toll receptor-like that leads to the production of reactive oxygen species. Sort Similarly, B. longum 51A was used to reduce the parasitic burden of Giardia in Mongolian gerbils (Meriones). unguiculatus), make this strain a preventative and therapeutic probiotic suitable for health promotion Human and animal transformed.
  • Methods: Preparation of microbial culture Bifidobacterium bifidum was obtained from the National Center for Genetic and Bio-Resource in Iran. Culture of Bifidobacterium Bifidum After regeneration of bifidobacterium bifidum lyophilized in MRS Broth medium Incubated at 30°C for 24 hours. the Continuing Work Case Use is given. Also, fresh culture was prepared for storage of bacteria. Bacteria Storage For long-term storage of bacteria for this research, stock is prepared and Periodically slaughtered. Fresh bacteria mixed with glycerol 50% for preparation of stook. It was then stored in vials at -20°C Detection of antibacterial properties of metabolites Isolation of metabolite from Bifidobacterium bifidum Isolation of antimicrobial metabolites from Bifidobacterium bifidum culture It was just made. The bacteria were then inoculated into the new medium and the bacteria were heated for 72 hours. Placed. Then, the medium was centrifuged with 12000 g for 10 minutes, then supernatant Isolated from cells and from supernatant cells for evaluation of antimicrobial and anticancer activity was used. For this purpose, supernatant of cells were lyophilized and then when working the concentrations of the cells were met. different from them newly developed. Culture of bacteria tested In this study, to investigate the antibacterial effect of Bifidobacterium bifidum metabolite from bacteria Bacillus anthracis، Proteus bacteria، Pseudomonas aeruginase، Klebsiella and Escherichia coli used. In the first stage, these three bacteria were cultured in liquid nutrient broth medium to prepare fresh bacteria. These bacteria were then used to investigate the antibacterial properties. Evaluation of antibacterial properties To investigate the antibacterial effect of Bifidobacterium bifidum in this study from Muller culture medium Hinton Agar was used. For this purpose, we use the method of creating wells to determine the amount of inhibitory is used. In wells method, we use suspension of pathogenic bacteria cultivated in the medium Nutrient broth (0.5 McFarland) was cultured with sterile swaps on Muller Hinton agar medium. then Sterile Pasteur pipette was used to create wells on the medium and made of Bifidobacterium supernatant liquid Bifidum with 0.1 ml concentration of 5, 10, 15 and 20 in wells and plates poured At 37 °C, it was stored for 24 hours. Measuring the diameter of the inhibition zone Then, the diameter of the inhibition zone created by Bifidobacterium bifidum against each of the bacteria Pathogenesis, Bacillus anthracis, Proteus and Pseudomonas aeruginase were measured.
  • Results: Antibacterial effect The diameter of inhibition zone was measured after the metabolite exposure to the three studied bacteria. In Table 1-4, the results of the inhibition zone of all three bacteria are presented. In this study, the supernatant was first prepared for bifidu bacteria 24, 48, 72 and was used for the method. Antibacterial was used for the studied bacteria and the Mullerington agar medium was used. Was. The results of this study showed that 72-hour supernatant has the highest effect and antibacterial properties The other two times were 24 and 48 hours. That's why these metabolites are lyophilized and It was used to continue. The effect of inhibition zone of bacteria in the vicinity of metabolite under study Bacteria mean diameter of inhibition zone (ml) Bacillus anthracis 12 Proteus 13 Pseudomonas aeruginase 3 Klebsiella Escherichia coli - Inhibition of growth by metabolite tested. These results show that the metabolites prepared in this study have the greatest effect on bacteria Proteus bacteria showed no growth inhibition effect on Klebsiella and Escherichia coli. Conclusion: Today, one of the important health problems is resistance of bacteria to various antibiotics. And it's been hard for researchers to always look for new materials with antibacterial properties to worry about. The public will be reduced in this case. What can be done in new investigations for anti-virus Bacterial metabolites are bacteria that can be effective in this regard. Hopefully they will be able to treat antibiotic-resistant bacteria.
  • Conclusion: It is believed that many intestinal bacteria are due to their metabolic properties and ability In changing microbial homeostasis, they are involved in various inflammatory and immune processes that affect the cause of the tumor. Although many of the microbiota functions are still unclear, across the spectrum Extensive intestinal microorganisms, various species belonging to the genus Bifidobacterium The results of this study showed that the metabolite of Bifidobacterium has antibacterial properties. So this metabolite can contribute to human health.
  • Keywords: Bifidobacterium bifidom, Antibacterial, Pathogen, Infection, Treatment