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E-Book 3rd Congress

  • Identification of the most important cause of stomach cancer by non-invasive method through oral cavity
  • Siavash Sharifi,1 Farnaz Farzaneh Dehkordi,2,*
    1. Department of Biology, Ardabil Branch, Islamic Azad University, Ardabil, Iran
    2. Department of Biology, Ardabil Branch, Islamic Azad University, Ardabil, Iran


  • Introduction: Helicobacter pylori is a gram-negative, microaerophilic, spiral-shaped bacterium that lives in the gastrointestinal tract and causes ulcers and cancer. This bacterium has been observed in the mouth in recent studies, so the mouth is its second reservoir. Endoscopic diagnostic test, Serology test, Stool test, Urease Breath Test and Polymerase Chain Reaction test are used to identify H.pylori. In the mentioned tests, samples taken from the stomach, blood, etc. are used. These tests have drawbacks and problems such as being aggressive, expensive, creating false results, and so on. The main purpose of this study was to evaluate H. pylori infection through saliva by PCR method as a fast, simple, cheap and non-invasive method and compare it with UBT (Gold Standard Test).
  • Methods: In this study, saliva samples were collected from 81 women and 63 men referred to Ardabil diagnostic laboratories with gastrointestinal symptoms suspected of H.pylori infection. DNA purification from case-control samples was performed by two methods of boiling and DNP sinaclon kit. After quantification and DNA quality assay by nanodrop to detect H.pylori, PCR was performed with specific primers (UreC) and the results were analyzed by SPSS software was performed under Chi-square test.
  • Results: Significance level was considered 0.05 and considering (P-value = 0.000) in comparing the results of PCR test through saliva with UBT test, we conclude that there is no significant difference between the results of the two tests and the relationship Strong prevails between them.
  • Conclusion: The results showed that this test has high sensitivity and specificity.
  • Keywords: stomach cancer, non-invasive method, oral cavity, h.pylory, PCR